| 000 | 02417nam a2200337Ia 4500 | ||
|---|---|---|---|
| 001 | 7984 | ||
| 005 | 20240925154104.0 | ||
| 008 | 190513?DAT1 CNT 000 0 LNG d | ||
| 020 | _a0879697725 | ||
| 040 |
_aUISEK-EC _bspa _erda |
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| 082 |
_a576 _bS187c 2006 |
||
| 100 |
_aSambrook, Joseph _916640 |
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| 245 |
_aThe condensed protocols : _bfrom molecular cloning : a laboratory manual / _cJoseph Sambrook, David W. Russell |
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| 264 |
_aNew York : _bCold Spring Harbor Laboratory Press, _c2006 |
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| 300 |
_a800 páginas : _bilustraciones ; _c26 cm |
||
| 336 | _atxt | ||
| 337 |
_2rdamedia _an |
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| 338 |
_2rdacarrier _anc |
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| 505 | _a1. Using Plasmid Vectors in Molecular Cloning 2. Bacteriophage λ and its Vectors 3. Working with Bacteriophage M13 Vectors 4. Working with High Capacity Vectors 5. Gel Electrophoresis of DNA and Pulsed Field Agarose Gel Electrophoresis 6. Preparation and Analysis of Eukaryotic Genomic DNA 7. Extraction, Purification and Analysis of mRNA from Eukaryotic Cells 8. In Vitro Amplification of DNA by the Polymerase Chain Reaction 9. Preparation of Radiolabeled DNA and RNA Probes 10. Working with Synthetic Oligonucleotide Probes 11. Preparation of cDNA Libraries and Gene Identification 12. DNA Sequencing 13. Mutagenesis 14. Screening Expression Libraries 15. Expression of Cloned Genes in Escherichia coli 16. Introduction of Cloned Genes into Cultured Mammalian Cells 17. Analysis of Gene Expression in Mammalian Cells 18. Protein Interaction Technologies | ||
| 520 | _aMolecular Cloning: Condensed Protocols is a single-volume adaptation of the three-volume third edition of Molecular Cloning: A Laboratory Manual. The book contains only the step-by-step portions of the protocols, accompanied by the appendices. Each protocol is cross-referenced to the appropriate pages in the original book. This affordable volume, designed for bench use, offers individual investigators the opportunity to have their own personal collection of short protocols from the world′s best-selling manual of molecular biology techniques. Índice | ||
| 526 | _aIngeniería Ambiental / Medicina | ||
| 650 | 0 |
_aBiología molecular _2lemb _96605 _xManual |
|
| 650 | 0 |
_aClonación _2lemb _91760 _xMolecular |
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| 901 | _a2011-07-01 17:11:40 | ||
| 902 | _a4 | ||
| 903 | _a2 | ||
| 904 | _aY | ||
| 905 | _aN | ||
| 942 | _cBK | ||
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