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The condensed protocols : from molecular cloning : a laboratory manual / Joseph Sambrook, David W. Russell

Por: Tipo de material: TextoTextoNew York : Cold Spring Harbor Laboratory Press, 2006Descripción: 800 páginas : ilustraciones ; 26 cmTipo de contenido:
  • texto
Tipo de medio:
  • no mediado
Tipo de soporte:
  • volumen
ISBN:
  • 0879697725
Tema(s): Clasificación CDD:
  • 576 S187c 2006
Contenidos:
1. Using Plasmid Vectors in Molecular Cloning 2. Bacteriophage λ and its Vectors 3. Working with Bacteriophage M13 Vectors 4. Working with High Capacity Vectors 5. Gel Electrophoresis of DNA and Pulsed Field Agarose Gel Electrophoresis 6. Preparation and Analysis of Eukaryotic Genomic DNA 7. Extraction, Purification and Analysis of mRNA from Eukaryotic Cells 8. In Vitro Amplification of DNA by the Polymerase Chain Reaction 9. Preparation of Radiolabeled DNA and RNA Probes 10. Working with Synthetic Oligonucleotide Probes 11. Preparation of cDNA Libraries and Gene Identification 12. DNA Sequencing 13. Mutagenesis 14. Screening Expression Libraries 15. Expression of Cloned Genes in Escherichia coli 16. Introduction of Cloned Genes into Cultured Mammalian Cells 17. Analysis of Gene Expression in Mammalian Cells 18. Protein Interaction Technologies
Resumen: Molecular Cloning: Condensed Protocols is a single-volume adaptation of the three-volume third edition of Molecular Cloning: A Laboratory Manual. The book contains only the step-by-step portions of the protocols, accompanied by the appendices. Each protocol is cross-referenced to the appropriate pages in the original book. This affordable volume, designed for bench use, offers individual investigators the opportunity to have their own personal collection of short protocols from the world′s best-selling manual of molecular biology techniques. Índice
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Tipo de ítem Biblioteca actual Colección Signatura Copia número Estado Fecha de vencimiento Código de barras
Libro Libro Miguel de Cervantes Sala general Col. General 576 S187c 2006 (Navegar estantería(Abre debajo)) Ej.1 Disponible 00010842
Libro Libro Miguel de Cervantes Sala general Col. General 576 S187c 2006 (Navegar estantería(Abre debajo)) Ej.2 Disponible 00010843

1. Using Plasmid Vectors in Molecular Cloning
2. Bacteriophage λ and its Vectors
3. Working with Bacteriophage M13 Vectors
4. Working with High Capacity Vectors
5. Gel Electrophoresis of DNA and Pulsed Field Agarose Gel Electrophoresis
6. Preparation and Analysis of Eukaryotic Genomic DNA
7. Extraction, Purification and Analysis of mRNA from Eukaryotic Cells
8. In Vitro Amplification of DNA by the Polymerase Chain Reaction
9. Preparation of Radiolabeled DNA and RNA Probes
10. Working with Synthetic Oligonucleotide Probes
11. Preparation of cDNA Libraries and Gene Identification
12. DNA Sequencing
13. Mutagenesis
14. Screening Expression Libraries
15. Expression of Cloned Genes in Escherichia coli
16. Introduction of Cloned Genes into Cultured Mammalian Cells
17. Analysis of Gene Expression in Mammalian Cells
18. Protein Interaction Technologies

Molecular Cloning: Condensed Protocols is a single-volume adaptation of the three-volume third edition of Molecular Cloning: A Laboratory Manual. The book contains only the step-by-step portions of the protocols, accompanied by the appendices. Each protocol is cross-referenced to the appropriate pages in the original book.

This affordable volume, designed for bench use, offers individual investigators the opportunity to have their own personal collection of short protocols from the world′s best-selling manual of molecular biology techniques.
Índice

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